3.2.3. Elimination of background fluorescence
Because the polynomial curve originally fitted to the whole
spectrum included regions with significant Raman peaks and
regions with only background fluorescence, it overestimated the
background signal (see Figs. 7 and 8). An improved estimate can be
obtained by fitting the curve to regions that the user selects as not
containing significant Raman peaks or by applying an automated
algorithm (e.g. Modified Polyfit [31], I-ModPoly [32]) designed
to ignore significant peaks. Since user selection can be slow and