After hybridization, the MNP-2pDNA/tDNA was washed twice with the assay buffer, then resuspended in 200 L assay buffer.
Meanwhile, the functionalized 1pDNA-AuNP-bDNA-NTs complex was centrifuged at 13,000 rpm for 20 min and the unreacted thiolated oligonucleotides in the supernatant were removed.