Directions
1. Dissolve 27.6 g of the medium in one liter of purified water.
2. Allow powder to soak for 10 minutes.
3. Heat with frequent agitation to completely dissolve the medium.
4. Autoclave at 121°C for 15 minutes.
5. Cool medium to 45 - 50°C and aseptically add 50 mL of lysed horse blood and 10 mL of ethanol
containing 20 mg of cefoperazone, 50 mg of cycloheximide, 20 mg trimethoprim, and 20 mg vancomycin.