This cross- sectional study was based on participants from an ongoing project- the Cholangiocarcinoma Screening and Care Program (CASCAP).Participantswere followings: 40 years or older, ever been infected with liver fluke, ever been treated with PZQ. PDF cholangiocawas defined by ultrasonography.Multiple logistic regression was performed to investigate therelationship amongnumber of repeated PZQ treatment,PDF and other confounder factors