T. tuberculata is capable of producing and releasing a large number of
water soluble and volatile toxic compounds into the soil and thereby
may also affect the growth of other plants in the vicinity. The incorporation
of dry leaf could increase weed biomass reduction and may decrease
the use of synthetic herbicides. Hence, this offers a promising
source of eco-friendly natural herbicide for sustainable agriculture.
However, additional studies are required to: a) validate the present
results on T. tuberculata under actual paddy field conditions, in order
to provide applicable recommendations to farmers and using the
allelochemicals produced by T. tuberculata as structural leads for developing
future new herbicides.Moreover, experiments are also needed to
establish the appropriate growth stage and agronomic and environmental
conditions underwhich this plantwill producemaximumquantities
of allelochemicals
tissues. Moreover, it can be useful to determine the phytotoxic interference
mechanism on receiver plants. GC–MS analyses of the different
plant parts (stem and leaf) of T. tuberculata revealed the presence of