The protease at a concentration of 0.20 mg mL1 was incubated
at 40 C with commercially available detergents: Ala (Protec &
Gamble); Bem-te-vi (Alimonda); Omo Multi-Ação (UniLever)
and Surf (UniLever) to a final concentration of 7 mg of detergent
mL1. Samples (150 lL) were removed at 10 min intervals (total
period of 60 min). The residual proteolytic activity in each
sample was determined at 25 C, assayed and compared with the
control sample incubated at 40 C without detergent pH 11.0