once ingested by the mosquito, the DENV establishes a productive infection in the mosquito midgut, where from the virus disseminates and replicates in other tissue.
In order to be transmitted to a human (or non human primate ) host durring the next blood meal, the DENV must ultimately infect the salivary glands and be shed in the saliva. Vector competence is genetically determined, and genetic traits influencing both midgut infection and escape barriers have been mapped to various loci on the Ae.aegypti chromosomes