The amp gene codes for a surface membrane protein that was
recently reported as being involved in insect-to-phytoplasma
transmission. Therefore, it is also suitable for phytoplasma strain
differentiation (4,28). Direct PCR assays with Amp-N1/C1
primers, which amplify 702 bp of the amp gene, were carried out
according to reported procedures (29). The 44 oil-palm samples
tested and the negative and positive controls were all as described
above. RFLP profiles generated with TruI and Tsp509I were
compared with those of the reference strains (Table 1). Direct
sequencing and sequence assembly were performed on the
amplicon from sample OP47. A phylogenetic tree was produced
using available reference strains (Table 1), as described above.
The full sequence of the amp gene was also analyzed with
translated nucleotide query, using BLASTP (version BLASTP
2.2.18; NCBI) (Table 3).
The amp gene codes for a surface membrane protein that wasrecently reported as being involved in insect-to-phytoplasmatransmission. Therefore, it is also suitable for phytoplasma straindifferentiation (4,28). Direct PCR assays with Amp-N1/C1primers, which amplify 702 bp of the amp gene, were carried outaccording to reported procedures (29). The 44 oil-palm samplestested and the negative and positive controls were all as describedabove. RFLP profiles generated with TruI and Tsp509I werecompared with those of the reference strains (Table 1). Directsequencing and sequence assembly were performed on theamplicon from sample OP47. A phylogenetic tree was producedusing available reference strains (Table 1), as described above.The full sequence of the amp gene was also analyzed withtranslated nucleotide query, using BLASTP (version BLASTP2.2.18; NCBI) (Table 3).
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