Preparation of Talk Based Formulation of Biocontrol
Agent: A loop full of S. griseus was inoculated in to the
nutrient broth medium and incubated for seven days
at 30°C and at 125 shakes/min. (Remi - C 24). After
incubation the broth containing 9 x 108 cfu/ml was used
for the preparation of talc based formulation. To 400 ml of
mycelial or spore suspension 1 kg of talc powder, calcium
carbonate 15 g (to adjust the pH to neutral and carboxy
methyl cellulose 10 g (as additive) were mixed under
sterile condition following the method described by
Vidhyasekaran and Muthamilan [7]. The product was
shade dried to reduce the moisture content to 20% and
then packed in polypropylene bags and sealed [8].