In January 2007, then ranked No. 212 in the world, Tsonga received a wild card entry into the 2007 Australian Open, where in only his second senior Grand Slam tournament match, he met sixth seed Andy Roddick for the second time in his career. What followed was the longest tiebreak in Australian Open history in the first set, which he went on to win (20–18). Tsonga forced a tiebreak in the second set, as well. However, he went on to lose the match in four sets. He was just 21 at the time.
In 2007, he won four Challenger titles in Tallahassee, Mexico City, Lanzarote, and Surbiton. Tsonga qualified for the 2007 Queen's Club Championships, while at the same time playing in the Surbiton Challenger, which he won. Between the two events, he won five matches during the course of two days. In the second round of the Queen's main draw, he met the sixth seed and defending champion, former World No. 1 Lleyton Hewitt, ranked No. 16 in the ATP rankings. Tsonga won the match after two tiebreaks to seal his most prominent victory since his triumph in ATP debut over former World No. 1 Carlos Moyà, then ranked No. 6 in the world, at Beijing in 2004. Suffering from fatigue, however, Tsonga went on to lose to promising Croatian youngster Marin Čilić in the following round.
At Wimbledon 2007, where he was again awarded a wildcard entry, he reached the fourth round (his first time past round one of a Grand Slam), defeating countryman Julien Benneteau, Nicolás Lapentti, and Feliciano López. His run was halted by his countryman and friend, 12th seed Richard Gasquet, in straight sets. He did not beat a seeded player in his progress to the fourth round (Andy Murray, the potential seed he would have faced, had dropped out). The win brought his ranking up from No. 110 to No. 74, his first time inside the top 75.
Then, at the 2007 US Open, Tsonga defeated Óscar Hernández before beating Tim Henman in what proved to be Henman's last Major.[8][9][10] He then lost to Rafael Nadal in three sets.[11]
The 2007 Grand Prix de Tennis de Lyon tournament started strongly for Tsonga. He beat Vince Spadea,[12][13] Richard Gasquet,[14] and Olivier Rochus, before losing to compatriot Sébastien Grosjean. He partnered Grosjean, however, in men's doubles, where the team was awarded a wildcard. They beat top seeds Julien Benneteau and Michaël Llodra, Fabrice Santoro and Gilles Simon, and then third seeds Arnaud Clément and Nicolas Mahut in the semifinals. Tsonga won his first doubles title, and Grosjean won his first doubles title in three years by defeating Łukasz Kubot and Lovro Zovko in straight sets.
By the end of the year, Tsonga saw his ranking rise over 150 ranking spots into the top 50. Tsonga began 2007 ranked No. 212 in the world, and in early July was in the top 100 at No. 74. In October, Tsonga climbed into the top 50 for the first time in his career, finishing the year ranked no. 43 in the world. Tsonga's year-end 169 ranking spots climb was the biggest climb of any player ranked in the top 75.