Phlebopus portentosus was isolated from a natural mature basidiome
purchased from a roadside stall in Amphur Weing-Par-Pao in Chiang Rai Province. It was originally
collected in a Fagaceae forest along a local roadside. The
isolation method basically followed Brundrett et al. (1996).
The basidiome was briefly brushed to remove adhering soil
particles, broken open, and a small piece of tissue was
removed with sterilized fine forceps and placed on solid
Murashige and Skoog (MS) medium at pH 4 in a Petri dish
(Straatsma et al. 1986). The pure culture isolate, designated
as P. portentosus WPPH2, was used for all experiments.
Mycelial cultures were maintained on MS medium at 20C
and were routinely subcultured every 2 months.