All the samples were selected from the soil treated with the organic (animal manure) and inorganic fertilizers (urea). Samples were collected throughout from spring to summer for continuous monitoring of soil microbial biomass and DNA contents. ODR of selected sites was also continuously monitored. All the samples were collected from the surface (0-20 cm) soil and were shifted to laboratory. Then soil samples were preserved at –20℃ for further analyses. Samples were later analyzed after sieving.