In this study, we used ethanol extract of A. princeps and investigated its antibacterial effects against MRSA. Ethanol extract of A.
princeps significantly inhibited MRSA growth and organic acid production during glucose metabolism at concentrations greater
than 1mg/mL (P < 0.05). MRSA biofilmformation was observed using scanning electron microscopy (SEM) and safranin staining.
A. princeps extract was found to inhibit MRSA biofilm formation at concentrations higher than 2mg/mL significantly (P < 0.05).
Bactericidal effects of the A. princepswere observed using confocal laser microscopy,which showed that A. princeps was bactericidal
in a dose-dependent manner. Using real-time PCR, expression of mecA, an antibiotic-resistance gene of MRSA, was observed,
along with that of sea, agrA, and sarA. A. princeps significantly inhibited mecA, sea, agrA, and sarA, mRNA expression at the
concentrations greater than 1mg/mL (P < 0.05). The phytochemical analysis of A. princeps showed a relatively high content of
organic acids and glycosides. The results of this study suggest that the ethanol extract of A. princeps may inhibit proliferation, acid
production, biofilm formation, and virulence gene expressions of MRSA, which may be related to organic acids and glycosides, the
major components in the extract.
In this study, we used ethanol extract of A. princeps and investigated its antibacterial effects against MRSA. Ethanol extract of A.princeps significantly inhibited MRSA growth and organic acid production during glucose metabolism at concentrations greaterthan 1mg/mL (P < 0.05). MRSA biofilmformation was observed using scanning electron microscopy (SEM) and safranin staining.A. princeps extract was found to inhibit MRSA biofilm formation at concentrations higher than 2mg/mL significantly (P < 0.05).Bactericidal effects of the A. princepswere observed using confocal laser microscopy,which showed that A. princeps was bactericidalin a dose-dependent manner. Using real-time PCR, expression of mecA, an antibiotic-resistance gene of MRSA, was observed,along with that of sea, agrA, and sarA. A. princeps significantly inhibited mecA, sea, agrA, and sarA, mRNA expression at theconcentrations greater than 1mg/mL (P < 0.05). The phytochemical analysis of A. princeps showed a relatively high content oforganic acids and glycosides. The results of this study suggest that the ethanol extract of A. princeps may inhibit proliferation, acidproduction, biofilm formation, and virulence gene expressions of MRSA, which may be related to organic acids and glycosides, themajor components in the extract.
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