Ultraviolet B (UVB) radiation induces photoaging by
upregulating the expression of matrix metalloproteinase (MMP) and
decreasing collagen synthesis in human skin cells. This study
evaluated the effects of Hesperethusa crenulata’s bark extract on
cytotoxicity and phototoxicity in human skin cells, MMP-1 and type I
pro-collagen levels in UVB-irradiated human skin fibroblasts by
ELISA. Powdered H.crenulata extracted with dichloromethane. The
toxicity showed in term of percentage of cell viability and the
morphology of cells was observed under microscope. Percent of cell
viability was compared with control (non-treat). The results indicated
that this extract didn’t show both cytotoxicity in human skin
fibroblasts and phototoxicity in human skin keratinocytes at the range
of dose that used in this study. For morphology, both of cells didn’t
change after were treated with this extract. It was found that
H.crenulata extract reduced MMP-1 expression and increased type I
pro-collagen production.