Differentially expressed proteins
Identification of differentially expressed proteins was carried
out as reported in [24] . In order to identify differences specifically
caused by a certain growth rate, proteins expressed in batch cul-ture and retentostats were compared to each other for a specific
substrate condition (acetate or acetate plus benzoate) via one-way
ANOVA with subsequent application of the t-test. False discovery
rates (FDR) were calculated as described in [24] . Two technical
replicates were taken from the retentostat experiments. In order
to gain sufficient power to recognize differentially abundant pro-teins, the randomization of the protein abundances was applied
100 times within each technical sample and not between the con-ditions. The FDR threshold was set to 2%.