Six sterile test
tubes with 9 ml sterile nutrient broth were taken. 1ml
of different concentration of drug solution was added
and 0.1ml inoculum was also added to the test tube
aseptically and media blank with the nutrient broth and
the drug solution was also prepared. A positive
control, containing media with 0.1ml inoculum was
maintained to indicate the growth promotion capacity
of the media. Test samples of Staphylococcus
epidermidis were incubated at 37°C for 24 hours and
those of Propionibacterium acnes were incubated
under anaerobic condition in an anaerobic jar (Hi-
Media) with gas pack for 48h.