This
observation may reflect the presence of L. garvieae carriers
among fish that will develop the disease only under detrimental
environmental conditions, as already suggested (1). The data
presented above show that the PCR assay performed on a very
limited amount of plasma (1 ml) is sensitive enough to detect
specifically L. garvieae in fish originating from a pond where an
active epizootic is occurring (Fig. 2a, lanes 1 to 4). In addition,
the PCR assay could detect carriers of L. garvieae in a pond
where the disease had occurred a previous year (Fig. 2a, lane
4). From these data, it can be concluded that the proposed
PCR assay can be useful not only for diagnostic but also for
epidemiological surveys and could be an efficient tool to establish
preventive measures on time (antibiotic prophylaxis,
vaccination).
This
observation may reflect the presence of L. garvieae carriers
among fish that will develop the disease only under detrimental
environmental conditions, as already suggested (1). The data
presented above show that the PCR assay performed on a very
limited amount of plasma (1 ml) is sensitive enough to detect
specifically L. garvieae in fish originating from a pond where an
active epizootic is occurring (Fig. 2a, lanes 1 to 4). In addition,
the PCR assay could detect carriers of L. garvieae in a pond
where the disease had occurred a previous year (Fig. 2a, lane
4). From these data, it can be concluded that the proposed
PCR assay can be useful not only for diagnostic but also for
epidemiological surveys and could be an efficient tool to establish
preventive measures on time (antibiotic prophylaxis,
vaccination).
การแปล กรุณารอสักครู่..
