Storage roots are an ecologically and economically important plant trait that have been independently derived multiple times in angiosperms. The primary function of these roots is long-term starch storage. These roots are characterized as having an accumulation of starch in tissue known as the anomalous cambium in the primary root and/or one or more lateral roots. In morning glories, storage roots were either gained or lost ten independent times. We have been investigating gene expression in storage roots (SRs) and fine roots (FRs) sampled from SR forming/non-SR forming species pairs. Anatomical assessment of starch accumulation and xylem location was done for all species included in the comparative RNA Seq study. Starch accumulation in SRs is similar among all SR-forming species. Xylem organization is similar in sweet potato, Ipomoea batatas, and a related species, I. lindheimeri but differs in the outgroup, Merremia dissecta. These results indicate that there have been two independent origins of storage roots in morning glories. Analysis of gene expression profiles revealed many more genes upregulated in SRs compared to FRs in both sweet potato and M. dissecta. Furthermore, fifty-seven orthologous genes were differentially expressed (DE) between SRs and FRs in both SR-forming species. These orthologous DE genes corresponded to many genes we expect to be involved in storage root formation, such as those in starch biosynthesis and alpha-expansin genes, and seven were transcription factors. Taken together, these results suggest that storage roots evolved independently at least two times in morning glories but utilized overlapping developmental pathways.