phospholipids and others like
polyanionic heteropolysaccharides (containing covalently-linked
hydrophobic side chains or complexes containing both
polysaccharides and proteins). In this study two unique isolates
L4 and L15 (screened from soil contaminated with oil) are grown
on MSM broth with 2% glucose for the production of biosurfactant.
Bio-surfactant was purified by Ammonium sulphate
precipitation or ZnCl2 precipitation method and identified by
TLC.