The treated leaves samples were soaked in solutions
of biologically active substances of plant origin -
heteroauxin (20 μg/ml) and zircon (1 μg/ml). In the solution
of these substances the leaves were rinsed for 20 minutes.
The samples of leaves were cut into small pieces, placed
in tubes with sterile distilled water (10 ml) and incubated
for 1 hour at 28°C, stirring occasionally. The resulting
suspensions were serially diluted with sterile distilled
water at a ratio of 1:100 and 1:1000, then plated on agar
selective media by traditional method and incubated at
28°C for 3 weeks