2.2. Essential oil content
At the end of the vegetative cycle, the lemon grass plants were
harvested early in the morning (from 7:00 to 10:00 am) and then
separated into aerial and root portions.
The plants were obtained from all treatments and analyzed.
From each treatment, 100 g of the fresh aerial parts of the lemon
grass plants were submitted to hydrodistillation (with 1 L deionized
water)in a modified Clevenger apparatus. The distillation time
was 2 h. The EO was removed with hexane, filtered with anhydrous
Na2SO4 and stored in amber flasks at 4 ◦C (Santos et al., 2009). The
content (%) was obtained after the solvent evaporation.