according to the respective OECD guideline 471 (OECD, 1997a) as
previously described (Roemer et al., 2012).
Determinations were performed in the presence and in the
absence of a metabolic activation system.
2.7. In vitro mammalian cell genotoxicity
Genotoxicity of TPM was assessed in the Mouse Lymphoma TK
Assay (MLA) developed by Clive et al. (1972). It was performed
according to OECD guideline 476 (OECD, 1997b) in the microtiter
plate version (Cole et al., 1986) with L5178Y cells with and without
metabolic activation as described in detail by Schramke et al.