Samples of PPW were supplied to the Biotechnology Laboratory
of the National Agricultural Research Foundation (N.AG.RE.F.) in
Greece and were dried. Moisture was determined by oven drying
at 105 C to a constant weight. Total sugars and total carbohydrates
were estimated by the colorimetric method of Dubois
et al. (1956). Protein was estimated by the Kjeldahl method by
multiplying residual nitrogen (N) by 6.25. Fat was determined by
the Soxhlet method (AOAC, 1995). Heavy metal analysis was performed
by atomic absorption according to the standard methods
for examination of water and wastewater, (APHA, 1989). Starch was quantified with the Kit. Cat. No. 10207748035, Böehringer
Mannheim/R-Biopharm. Ethanol was measured with the Böehringer
Mannheim/R-Biopharm, Kit. Cat. No. 10 176 290 035. The
degree of polysaccharide degradation was estimated by quantifying
the amount of reducing sugars formed during enzymatic or
acidic hydrolysis. Reducing sugars were determined as glucose
by using dinitrosalicylic acid (DNS) reagent at optical density
575 nm, by the method described by Miller (1959).