Large scale production of a-amylase
using various Bacillus sp. and Aspergillus oryzae has been reported.
5 Bacillus sp. is an industrial important microorganism
because of its rapid growth rate, secretes enzyme into the
extracellular medium and safe handling.6 This study aims in
isolation, molecular characterization of native amylase producing Bacillus subtilis from the soil samples collected from
sago industry waste site and amylase production, optimization
conditions and partial purification of a-amylases using
cassava starch as carbon source also were studied. Nitrogen
sources, pH, temperature, substrate concentration, amino
acids, Inoculum concentration, incubation time and surfactants
have been optimized for enhanced production and they
play an incredible role in amylase production.