จากรูป 5a.) แสดงให้เห็นถึง Decolorization time-course of synthetic dyes (a) RBBR by immobilized or free laccase in the presence and absence of redox mediator (HBT, 1 mM). พบว่า Ca-alginate beads were able to remove about 12% of initial RBBR dye within 90 min, but the decolorization of RBBR is based on 1 mM of HBT of redox reactions decolorization of RBBR and the reaction of oxidation RBBR easy with Free laccase. only