In the experiment using the basic bacteriological transfer techniques and flame sterilization that is the techniques to sterilization bacteria. Using proper aseptic technique can greatly minimize the risk of contamination from environment. It is of importance to maintain pure stock cultures while transferring cultures to new media. Aseptic technique is also essential for isolation of a single species of microorganism from a mixed culture to obtain a pure culture. Aseptic technique consist of Flaming the loop, Flaming the Mouth of the Test Tube, Agar Slants that containing agar, clean hand and clean table. There are factors affect to this experiment that makes it error such as another microbes in laboratory, accidentally, infecting people working in the laboratory.