The degree of hydrolysis and the total nitrogen utilization rate of the mash incubated at 55c for 48 h were about 40%and 75% respectively.
Although the total nitrogen content was a littlehigher in the hydrolysate in incubated at 55c for 48 h the contents of formol nitrogen and amino nitrogen were both lower than those incubated at 45 c for 48 h.
These result indicated that rapid fermentation at 45c could obtain higher amounts of amino acid and short-chain oligopeptides in koji hydrolysate and conscquently could offer better flavor-enhancing contribution.