Fat extraction and Fatty Acids Determination
Fat from fresh milk, yogurt and concentrated yogurt was extracted using chloroform-methanol (2:1; v/v) and then converted
into fatty acids methyl esterase or FAME. Fatty acids profiles were determined using a gas chromatography [15]. The
individual fatty acid contents were expressed as weight percentages (g/100 g of fat). Grouping of fatty acids were as follow:
(1) saturated fatty acids (SFA), (2) short-chain saturated fatty acids (C4 to C10, SCFA