The optimization of culture conditions for the growth and antifungal activity of antagonistic bacteria is important for industrial applications.
Evaluation of nutritional and environmental requirements of microorganism is crucial for bioprocess development(Abdel-Fattah and Olama, 2002).
In addition, biochemical systemsare multivariable processes (Rajendran and Thangavelu, 2007), and therefore production of biological control agents by antagonistic bacteria should also be performed by several regulatory mechanisms
which respond to conditions in the culture environment.
We previously isolated an endophytic bacterium (BT4) from healthy tomatoes. BT4 has high antifungal activity against Alternaria solani, a pathogenic microorganism that causes early blight of tomato. Based on 16S rDNA sequence, BT4 was identified as
Bacillus amyloliquefaciens. In the present study, the optimal medium composition and culture conditions for BT4 were determined using the ‘‘one factor at a time’’ (OFAT) method and an orthogonal design.
The antifungal activity of BT4 for tomato storage was also tested.
The optimization of culture conditions for the growth and antifungal activity of antagonistic bacteria is important for industrial applications. Evaluation of nutritional and environmental requirements of microorganism is crucial for bioprocess development(Abdel-Fattah and Olama, 2002). In addition, biochemical systemsare multivariable processes (Rajendran and Thangavelu, 2007), and therefore production of biological control agents by antagonistic bacteria should also be performed by several regulatory mechanismswhich respond to conditions in the culture environment.We previously isolated an endophytic bacterium (BT4) from healthy tomatoes. BT4 has high antifungal activity against Alternaria solani, a pathogenic microorganism that causes early blight of tomato. Based on 16S rDNA sequence, BT4 was identified asBacillus amyloliquefaciens. In the present study, the optimal medium composition and culture conditions for BT4 were determined using the ‘‘one factor at a time’’ (OFAT) method and an orthogonal design. The antifungal activity of BT4 for tomato storage was also tested.
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