To obtain CPS,distilled water was added to carob pulp(1:2w/w) andthe extraction was carried outat room temperature, overnight. Afterwards, the mixture was pressed and squeezed. The supernatant centrifuged at 8900g for 20 min at 4C using an Avanti J-25 I centrifuge (Beckman Coulter, Fullerton, USA) and filtered with a 0.22lm membrane filter, in order to remove particles in suspension. In the same way, SCM were also centrifuged and filtered.