Up to date, there have been studies which reported the effects of agricultural practices on the secondary metabolites in medicinal and aromatic plants (Ozguven et al., 2002; Sekeroglu and Ozguven, 2006; Ashraf et al., 2006). The aim of the present study was to investigate the effects of different nitrogen doses (0, 40, 80 and 120 kg ha−1) on the yield and the artemisinin content of annual wormwood (A. annua L.)