Based on the above results, it could be concluded that the two milling process, which include different conditioning times and various clearance between rolls had a noticeable effect on the characteristics of wheat flour. On the other hand, the pan bread produced from flour of Gemmeiza 11 was found to have excellent quality grade for all the evaluated characteristics than bread produced from flour obtained from Sakha 94. It could be concluded that the flour obtained from wheat sakha 94 is unsuitable for production of pan bread with high volume due to its low damage starch percent and low enzyme activity which resulted in dough having low ability of gas retainment.