as determined
by cell density (n = 3). (B) Growth during 12 h:12 h light:dark cycle as determined by cell density (n = 2). Shaded regions denote dark periods. (C)
Growth rate of transgenic strains compared with WT during light:dark cycle. Shaded regions denote dark periods. L1, first light period; D1, first
dark period, etc. (D) Increased BODIPY fluorescence and TAG accumulation of 1A6 and 1B1 over WT in stationary phase. E, exponential phase; S,
stationary phase. Error bars are SEM from imaging flow cytometry (n > 100). Horizontal lines depict average of three graphed populations. (E)
Imaging flow cytometry images of representative cells selected because their BODIPY fluorescence per cell was the average of the population for
three replicates of WT, 1A6, and 1B1 in stationary phase. BODIPY and chlorophyll fluorescence (Chl) are both shown. (F) TAG levels in 1A6, 1B1 and WT
over light:dark cycle monitored using BODIPY fluorescence. Shaded regions denote dark periods. Error bars are SEM from imaging flow cytometry
(n > 100). Statistical analyses were performed using Student t test, *P < 0.05.