Testicular histology also showed the degenerative
changes associated with the graded doses of calcium
chloride. The disintegration of germ cell association in
seminiferous tubules and washing out of germ cells
from the seminiferous tubules were noted even with the
lowest dose, though the tubular compartment remained
distinct with respect to the extra-tubular compartment.
Drastic necrosis in seminiferous tubules along with atrophy
of the tubules was noted starting with the 10%
dose. But after the high dose (20%) of calcium chloride
treatment, complete degeneration of germ cells was
observed, together with the absence of a distinct boundary
of seminiferous tubules with respect to the interstitial
spaces along with appearance of fibrous tissue and
hyaline tissue. These changes may be due to the necrotizing
properties of calcium chloride as reported by
others [34-36]. In addition, germ cell degeneration by
calcium chloride has been associated with low serum
concentrations of testosterone, a prime regulator for the
maintenance of structural morphology as well as the
normal physiology of seminiferous tubules [37-39]