In this paper, HPLC and UPLC methods for vitamin C determination
were compared. AA and DTT were eluted from an HPLC system
in 4.01 and 13.06 min, respectively. Using UPLC system, retention
times of AA and DTT were 1.56 and 3.30 min, respectively
(Fig. 1). Using UPLC method, the total time of analysis was 2.5
times shorter than with HPLC and solvent consumption decreased
from 12 to 1.2 mL/min.