y. For preparation of
EHEC, 0.5 g of the curry leaf powder was accurately weighed in a
polypropylene centrifuge tube and then 10 ml of ethanol was
added to it, and the tube was held for 10 min at room temperature
(27 ± 1 C), vortexed at high speed for 10 min, and finally centrifuged (Elico, USA) at 5000 rpm for 10 min. Supernatant was
collected into a glass test tube, and the compounds were reextracted with 10 ml of the same solvent followed by centrifugation once again as above