The process temperature for buffalo met blocks
processed in retort pouches calculated based on the heat
resistance of Clostridium sporogenes PA 3679 in Phosphate
buffer saline (PBS- Ph 7.0) as reference medium and in
buffalo meat block (pH 6.28) was in the range of 110–
121°C. The D values and Z values calculated for C.sporogenes
PA 3679 confirmed that the suspension was best
suited for conducting thermal resistance studies. The experiment
for indirect confirmation of microbial safety of the
products involving inoculating the buffalo meat emulsion
filled in pouches with C.sporogenes PA 3679 and processed
at Fo 12.13 min showed no growth of microorganisms.