Partial substitution of cow milk with ewe or goat milk in pasta filata cheese production had an effect on physicochemical, microbiological, and senso- rial characteristics of the final products. The different compositions of raw milk contributed to the peculiar sensorial attributes of the innovative produced cheeses, also influenced by the native microbiota of the differ- ent milks. All innovative and traditional Caciocavallo cheeses showed a satisfactory nutritional, sensorial, and microbiological quality. Moreover, considering the peculiar nutritional properties of goat milk, the real- ized mixed goat Caciocavallo cheeses could lead to good success among consumers interested in healthy foods.