Likewise, in S. Typhimurium, amplified
products from the enriched cells in PED were detected much earlier
than the samples taken from stomacher bag, but much of the amplification
was noticed much later than E. coli. In fact, late amplification could
be caused by carry over contamination (Killgore et al., 2000); however,
according to a previous report, trace amounts of DNA can be detected
and appeared even after 34 cycles on the amplification plot, and the
late amplified product was confirmed by sequencing (Mothershed
et al., 2002).
Likewise, in S. Typhimurium, amplifiedproducts from the enriched cells in PED were detected much earlierthan the samples taken from stomacher bag, but much of the amplificationwas noticed much later than E. coli. In fact, late amplification couldbe caused by carry over contamination (Killgore et al., 2000); however,according to a previous report, trace amounts of DNA can be detectedand appeared even after 34 cycles on the amplification plot, and thelate amplified product was confirmed by sequencing (Mothershedet al., 2002).
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