Crude extract preparation
Bulbs of garlic were first peeled to make the drying process faster.
Bulbs of garlic were then kept in a shaded room for a period of 5 to
7 days for drying. The bulbs were first chopped followed by grinding
to obtain dried powder material. The powder were then placed in a
tank and methanol added to the tank till the powder material was
completely dipped in. Shaking of the tank for 7 to 10 days was
performed twice a day. The solution (methanol + extract) were then
subjected to the rotatory evaporator to separate methanol leaving
only semisolid extract solution (crude extract). For further study,
crude extract was fractioned with different solvents that is, butanol,
ethyl acetate, chloroform, petroleum ether and water.