Nevertheless, the advantages of in vitro culture in a liquid medium are often counterbalanced by technical problems such as asphyxia and hyperhydricity, a typi- cally stress-induced change observed in morphological, anatomical and physiological disorders [8,9]. Various procedures have been developed to avoid these problems [9]. These include the use of temporary immersion sys-tems (TIS) to improve in vitro growth and plant quality in different species [10]. But, few bamboo species such as Bambusa ventricosa [11], Guadua angustifolia Kunth [12], and Dendrocalamus latiflorus [13] have been propagated by TIS. The aim of this report was to deter- mine the optimal concentration of 6-Benzyladenine (BA) for Bambusa vulgaris shoot proliferation in a TIS, and to clarify whether the different concentrations of BA influ- ence during the multiplication. Here we present the first report of the successful propagation of B. vulgaris in this semi-automatic system