. The mass spectrum of the eluent also validated the presence of dimer (m/z 535.95).
The effect of extraction parameters on extraction efficiency including sorbent amount, elution conditions
(solvent type and volume) and adsorption conditions were investigated. Under the optimized conditions,
the linear dynamic range was found to be 0.05–5.00 g mL−1 with detection limit of 0.02 g mL−1. The
extractionefficiency was94%andthe relative standarddeviation was4%for three replicatemeasurements
of adenosine at 0.25 g mL−1 in urine samples. As a practical application, the method was applied for the
determination of adenosine in urine samples of patients with lung cancer, and the obtained results were
in good agreement with those obtained by HPLC–UV method. Therefore, the proposed method is an
alternative clinical analysis.
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