The growing interest in RS has led to the need for a valid
analytical method for an appropriate quantification of RS in
food and food products. RS has been defined as ‘the sum of
starch and products of starch degradation that are not
absorbed in the small intestine of healthy individuals’ (Asp,
1992). Thus, according to this definition the analytical
method of choice to quantify the RS content of foods
should measure all the starch and α-dextrins present.
Furthermore, this analytical method should be validated by
a direct comparison of the data obtained in vitro with the
true in vivo data from healthy subjects.