Se-BR was prepared as described previously (Liu et al., 2011).
Brown rice was germinated in the dark with 60 lmol/L sodium
selenite at 25 C for 3 day.
2.2. Determination of total Se and Se species in Se-BR
2.2.1. Determination of total Se
Approximately 0.5 g of freeze dried sample was placed in a centrifuge
tube and then added with 6 mL of concentrated HNO3 and
2 mL of 30% H2O2. After 16 h, the tubes were capped and placed in
a microwave oven. The power was raised to 90 C in 10 min, to
120 C in the next 10 min, and maintained at 120 C for 30 min.
A