The antioxidant capacity of coffee brew extracts was determined using oxygen radical absorbance capacity fluorescein (ORACFL) assays as described by Kitts and Hu (Kitts & Hu, 2005). Briefly, 100 lL serial concentrations of Trolox (0 0.001 mg/mL) or coffee brew extract (0.001 0.02 mg/mL) in 75 mM phosphate buffer (pH 7.0) or Trolox standards (finalconcentration, 0– 6.0 mM) and 60 lL of fluorescein (Sigma-Aldrich, St.