These macerated mashes were heated at 90°C for 5 min to stop the activity of enzymes and pH was readjusted to 5.0.
Subsequently, the pectinase-hydrolyzed mash was treated with 0.3 ml of cellulases at 60°C for 2 h. The other set of banana mash was treated with 0.3 ml of cellulases alone at 60°C. Then each set of the mashes was allowed to come to room temperature before the fermentation process was carried out. Untreated banana mash was used as a control for ethanol production.