Blood is the most frequently found evidence at the crime scene in one or the other form. Blood and its stains are quite often encountered in crime cases like murder, rape and assaults etc. They are required to be analyzed properly for their nature, species origin and individual characteristics. In an attempt to hide evidence, perpetrators can wash and destroy bloodstains, making them invisible to the naked eye and it is not known through which conditions the bloodstain has undergone before analysis. A wide variety of chemicals are used to affect the nature of bloodstains which poses lot of problems in the analysis of bloodstains. According to the nature of evidence different methods have been studied for the extraction of DNA.[1], [2] and [3] Once biological evidence has been identified, it is necessary to determine whether or not it is of human origin and if it is of non-human origin, then to what species it belongs. After determining the species origin, then it becomes necessary to determine to which blood group it belongs.