The ethanol product was prepared and assessed using the HPLC
methods described by Elliston et al. (2013). SSF samples were centrifuged,
filtered, measured using an HPLC fitted with carbohydrate
analysis column with RI detector (Elliston et al., 2013). Ethanol
yield (% w/w) was calculated according to the formula below.
Ethanol yield ð%Þ ¼
ethanol product ðgÞ
theoretical ethanol product ðgÞ
100%
The theoretical amount of ethanol product (g) was obtained
from molecular weight of glucose (180.2 g mol1) and ethanol
(46.1 g mol1). Thus, the theoretical ethanol product is 51.2%
(w/w) of the maximum glucose content of steam exploded slurry.
The maximum glucose content of steam exploded slurry have been
measured in the previous research (Zhao et al., 2015).