waste pastry was hydrolyzed by glucoamylase and protease which were obtained from solid state fermentation of aspergillus awamori and aspergillus oryzae to produce waste pastry hydrolysate. then the effects of hydraulic retention time on hydrogen production rate in the suspended microbial growth system from waste hydrolysate were investigated.The maximum HPRs of CSTR and CMISR were obtained at HRT of 6 hour and 4 hour,respectively.