3.2. Antioxidant activity
Even if some phenolic substances are destroyed or inactivated
during the hot extraction of coconut oil, there is a possibility that
the inactivated or oxidised phenolic compounds contribute to the
overall absorption during the colorimetric quantification of total
phenols. The inactivated or oxidised phenolic substances may also
appear in the phenolic region of the HPLC chromatograms. If this
happens, the colorimetric and chromatographic phenolic contents
may not reflect the antioxidant potentials of the phenolic extracts.
Therefore, both in vitro and in vivo antioxidant assays were carried
out to determine the antioxidant potentials of the phenolic extracts
of CECO and HECO.